The findings of several prior studies indicate high failure rate of Information systems implementation projects in different organizations. These studies also point to the fact that many of these failures are as a result of organizational issues as well as not paying attention to current situation of the organization in early stages of Information system development, rather than technical issues. In other words, successful implementation of an information system is directly depending on current situation of the organization. Considering high needed investment for implementing an information system project, it seems to be necessary to assess its current organizational readiness level. In this study, on the basis of a comprehensive reviewing various relevant models of readiness assessment for information systems development has been attempted to present a model about the existing realities and internal circumstances in public sector organizations and institutions emphasizing on universities and higher education institutions. Based on proposed model, to assess organizational readiness, six major dimensions (Strategic, Structural, Resources, Cultural, Managerial, and Legal) were identified from the literature which was divided into 47 sub-dimensions and 142 indicators. The identified indicators can be used to assess organizational readiness in order to facilitate a purposeful and appropriate information system development and consequently to prevent the waste of organizational resources. The proposed model also was used in a public university as a case with the aim of determination of organizational readiness level, and finally several action plans were suggested based on the obtained research results.