Today, government services or e-government is one of the most important issues in society. Since in the e-government environment, the knowledge management strategy plays a central role, this paper has aimed to study knowledge access, creation, and transfer in portals of Iran ministries. The statistical populations of this study consisted of all Iran ministries portals. An evaluation model known as knowledge access, creation and transfer (K-ACT) was presented and with using this model, a checklist was developed and applied for evaluating the portals. The findings indicated that, on average, ministries’ portals featured only about 26.4 percent of the knowledge access, 30 percent of knowledge creation, and 22 percent in knowledge transfer. The ranking of portals also showed that ministries of information and communication technology, and industry and mine in knowledge access economic affairs and finance, health and medical education, defense, and industry and mine in knowledge creation and the ministry of economic affairs and finance in knowledge transfer with 41 percent of criteria have won first place between the portals. Generally, the portals of economic affairs and finance, and industry and mine ministries with 42 percent and ministry of information and communication technology with 34 percent of criteria, had the highest standards in the field of knowledge management. The results indicated that there were many weaknesses in the use of new technologies - in particular, portals - in Iran that must to be examined and fixed by planning and scientific work and the use of various experts' opinions.